In contrast, cool vent water with low chemosynthetic activity yielded predominantly phylotypes related to freshwater Actinobacterial clusters with a cosmopolitan distribution. In-depth geophysical and geochemical exploration and lake floor mapping of Yellowstone Lake, the largest Alpine Lake in the United States, has revealed numerous sublacustrine hot vents and hydrothermal features in geothermally active areas on the lake bottom (Klump et al., 1988, 1995; Remsen et al., 1990; Morgan et al., 2003a, b).
Reduced sulfur compounds (hydrogen sulfide, thiosulfate, sulfite) were quantified by a scaled-up modification of the microbore high-performance liquid chromatographic (HPLC) method of Vairavamurthy and Mopper (1990), using dithio-bis-nitropyridine (DTNP) derivatization.
Samples were collected in PP syringes and after rinsing, exactly 10 m L were squeezed through 0.2 μm nylon syringe filters (Whatman Acrodisc) into acid-washed 20 m L liquid scintillation vials (LSV). nov., an extremely thermophilic, facultatively heterotrophic, sulfur- oxidizing bacterium from Yellowstone National Park, and emended descriptions of the genus Sulfurihydrogenibium, Sulfurihydrogenibium subterraneum and Sulfurihydrogenibium azorense.
Rubber-free all polypropylene (PP) syringes with three-way valves were used to collect water from the 2-L ROV-mounted piston syringe samplers without introducing headspace.
The ROV’s screw drive was used to squeeze the water into receiving syringes to maximally retain dissolved gases.
The ROV is fully operator-controlled with thrusters that allow for lake floor reconnaissance and positioning for sampling, and included live video for vent identification and guided sampling (Lovalvo et al., 2010); it is deployed from RV Cutthroat, the Yellowstone Lake research vessel owned and operated by the National Park Service. Temporal and spatial archaeal colonization of hydrothermal vent deposits.
Vent water samples were retrieved from the ROV collection syringes and put into smaller, all-plastic syringes through a three-way valve without exposure to air. Pubmed Abstract | Pubmed Full Text Page, A., Tivey, M.
The fifth sample from the West Thumb Basin, West Thumb 98, represents a cooler, low activity water sample from the West Thumb area.
In situ temperatures measured by ROV, and chemistry of the syringe-sampled waters are shown in Table 1.
Each analysis required 45 min in a dual mobile phase gradient separation on a 250-mm C18 5 μm HS reversed phase column (Alltech Assoc.) so as many as 30 samples could be analyzed daily.
Light and dark COC, as described previously (Cuhel et al., 2002).